Jon Fleetwood has reported a concerning discovery regarding Pfizer's COVID-19 mRNA vaccine (BNT162b2): its production plasmid contains human DNA fragments that regulate blood, immune, and neurological systems, the same systems most frequently associated with severe adverse events (AEs) post-vaccination. Independent studies, including a French government-funded study (Nov 2024) and a peer-reviewed paper in Science, Public Health Policy, and the Law (Dec 2024), discussed by Brian Simpson at the blog today, confirmed excessive plasmid DNA contamination in vaccine vials, with levels up to 516 times above regulatory limits. These findings, coupled with evidence of plasmid DNA integration into human cells, raise critical questions about whether the vaccine's design contributes to its safety signals. This essay outlines the evidence, the overlap between plasmid sequences and AE categories, and the urgent need for regulatory scrutiny.
Plasmid DNA Contamination in Pfizer's Vaccine
Pfizer's mRNA vaccine is synthesised using a DNA plasmid template, a circular DNA molecule engineered to produce mRNA encoding the SARS-CoV-2 spike protein. Plasmids are inherently integration-competent, widely used in genetic engineering to insert foreign DNA into genomes. Independent analyses have confirmed residual plasmid DNA in Pfizer's vaccine vials, including:
Dr. Didier Raoult's Study (Nov 2024): A French government-funded study found 5,160 ng of plasmid DNA per dose, 516 times higher than the FDA and EMA's 10 ng safety limit. The contamination included bacterial sequences (origin of replication, kanamycin resistance gene) and the SV40 promoter/enhancer, historically linked to oncogenesis.
McKernan et al. (Feb 2024): A preprint study detected plasmid-specific sequences, including the spike gene and SV40 promoter, integrated into the genome of human ovarian cancer cells (OVCAR3) in vitro, using qPCR and DNA sequencing. While limited to a cancer cell line, this demonstrated integration potential.
Buckhaults (Nov 2024): Dr. Phillip Buckhaults found persistent plasmid DNA (spike gene, SV40 promoter, NeoKanR gene) in the genomic DNA of normal human epithelial stem cells (colon organoids) one month after exposure, confirming integration in non-cancerous cells.
Science, Public Health Policy, and the Law (Dec 2024): This peer-reviewed study reported 227–334% higher DNA contamination than WHO limits, including SV40 sequences, and called for an immediate moratorium on mRNA vaccines due to integration and oncogenic risks.
These findings contradict claims by regulatory bodies like the TGA and FDA, which assert no evidence of genomic integration or harm from residual DNA. The presence of SV40 sequences is particularly concerning, as they can enhance gene expression and are linked to cancer in historical studies.
Human DNA Sequences in Pfizer's Plasmid
Pfizer's plasmid incorporates three human-derived untranslated regions (UTRs) to stabilize mRNA and enhance translation, as confirmed in an October 2023 Nature npj Vaccines publication:
1.α-globin 5′UTR (Blood/Cardiovascular): Derived from the human haemoglobin α-globin (HBA1) gene, this sequence stabilises mRNA during red blood cell development. In synthetic constructs, it boosts translation efficiency in mammalian cells, directly tying to cardiovascular biology.
2.AES/TLE5 3′UTR Fragment (Immune System): This fragment, from the AES/TLE5 gene family, encodes transcriptional co-repressors involved in immune signalling. It extends mRNA half-life and increases antigen production in antigen-presenting cells, amplifying immune activation.
3.MT-RNR1 Fragment (Neurological): Derived from mitochondrial 12S rRNA (MT-RNR1), this sequence is essential for mitochondrial protein synthesis. Variants are linked to neurological syndromes and hearing loss. In Pfizer's plasmid, it enhances mRNA stability, aligning with neurological functions.
These sequences are not random; they are regulatory codes drawn from human genes governing blood, immune, and neurological systems. If integrated into the human genome, they could disrupt these systems' regulation.
Adverse Events: A Striking Overlap
Two peer-reviewed reviews highlight the primary AE categories post-vaccination:
2022 Systematic Review (Archives of Academic Emergency Medicine): Synthesised 74 studies, identifying cardiac (myocarditis, pericarditis, tachyarrhythmias, clotting), immune/allergic (anaphylaxis, hypersensitivity, immune dysregulation), and neurological (Guillain-Barré Syndrome, Bell's palsy, cerebral venous sinus thrombosis, seizures) as the dominant serious AEs. Cardiac events were the most reported.
2024 Review (Pharmacology Research & Perspectives): Confirmed the same pattern, noting myocarditis (highest in young men post-Pfizer), immune dysregulation linked to lipid nanoparticles and PEG, and neurological complications like neuroinflammation and thrombosis.
These categories, cardiac, immune, and neurological, mirror the biological systems regulated by the plasmid's human DNA sequences. This alignment suggests that integration of these fragments could dysregulate the very systems they target, contributing to observed injuries.
Evidence of Genomic Integration
Several studies provide evidence that plasmid DNA and mRNA from Pfizer's vaccine can integrate into human DNA:
2022 Study (Current Issues in Molecular Biology): Demonstrated that BNT162b2 mRNA is reverse-transcribed into DNA in human liver cells (Huh7) within 6–48 hours via LINE-1 retrotransposons. While focused on mRNA, this supports the feasibility of nucleic acid integration.
2023 Nature Scientific Reports Study: Found that 10–20% of human cells transiently transfected with linear DNA became stably transfected, with junction sequencing confirming genomic integration.
2022 Review (Journal of Neurological Disorders): Outlined mechanisms for mRNA and plasmid DNA integration via LINE-1 retrotransposons and polymerase θ, noting that spike-induced DNA damage and mRNA stability (via methylpseudouridine) increase integration risk.
McKernan and Buckhaults Studies (2024): As noted, both confirmed plasmid DNA integration in cancer and normal human cells, respectively, with persistent sequences including the SV40 promoter.
These findings challenge the narrative that mRNA vaccines cannot alter DNA, as asserted by the TGA, WHO, and others. The presence of integration-competent plasmids, combined with excessive DNA contamination, raises the possibility that regulatory sequences could disrupt gene expression if integrated.
Implications and Motives
The overlap between the plasmid's human DNA sequences and AE categories is unlikely to be coincidental. Potential explanations include:
Design Oversight: The inclusion of α-globin, AES/TLE5, and MT-RNR1 UTRs was intentional to enhance mRNA stability and translation, as per Nature npj Vaccines. However, the risks of residual plasmid DNA integration were not adequately studied, reflecting rushed development during the pandemic.
Regulatory Failures: The FDA, EMA, and PMDA skipped critical safety tests (e.g., genotoxicity, carcinogenicity, biodistribution), misclassifying mRNA vaccines as traditional vaccines rather than gene therapies. This allowed high DNA contamination levels to go undetected.
Commercial Pressures: The rapid rollout, backed by liability shields, prioritised speed and profit over rigorous safety assessments, as critiqued in Science, Public Health Policy, and the Law.
These factors suggest systemic negligence. The SV40 promoter's presence, known for oncogenic potential, amplifies concerns about long-term risks like cancer.
The evidence demands immediate action:
Moratorium on mRNA Vaccines: The excessive DNA contamination (up to 5,160 ng/dose) and integration potential warrant halting Pfizer's vaccine until safety is proven.
Forensic Investigations: Regulators must analyse the full plasmid sequence, which Pfizer has not disclosed, and conduct in vivo studies to assess integration in vaccinated individuals.
Enhanced Surveillance: Tools like Neo7Bioscience's Spike X Detect can identify integration events and guide personalised therapeutics to mitigate harm.
Transparency and Accountability: Independent audits of regulatory processes and public disclosure of vaccine composition are essential to restore trust, if at all now.
Conclusion
Pfizer's mRNA vaccine contains human DNA fragments (α-globin, AES/TLE5, MT-RNR1) that regulate blood, immune, and neurological systems, precisely the systems most affected by severe AEs like myocarditis, immune dysregulation, and neurological disorders. Independent studies confirm excessive plasmid DNA contamination and its integration into human cells, challenging regulatory claims of safety. The striking overlap between the plasmid's design and AE categories suggests that integration may contribute to these injuries. With evidence mounting, regulators must halt mRNA vaccine use, investigate integration risks, and value public safety over commercial interests to prevent further harm.
https://jonfleetwood.substack.com/p/covid-19-mrna-shot-plasmids-contain